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人玻璃粘连蛋白溶液
起批量( 价格
≥1 ¥1元/瓶
  • 供货能力:1000
  • 最低订购量:1瓶
  • 可销售总数量:1000瓶
  • 建议零销价:1元/瓶
  • 发布日期: 2019-06-03
  • 更新日期: 2019-06-03
产品详细说明
品牌: 进口 产地:
CAS号: 英文名称: Human Vitronectin Solution
保质期: 保存条件:
货号:

Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types。 This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides。

Vitronectin’s primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.

Vitronectin is ideal for coating of surfaces。 The optimal concentration for cell attachment and culture may differ for various cell types。 Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware。 It is provided in user-friendly packaging for use and storage。 Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment。 This product is shipped separately on dry ice。

Parameter, Testing, and Method

Vitronectin
Catalog # 5051-0.1MG

Quantity

0.1 mg

Volume

0.2 ml

Concentration

0。5 mg/ml

Purity

>95%
as measured by Coomassie stain

Formulation

0.15 M NaCl, 0.005M HEPES at pH 7.4

Form

Solution

Source

Human, plasma

Storage Temperature

-20°C

Shelf Life

12 months after receipt

Sterilization Method

Filtration

Cell Attachment Activity

Passes

Sterility

No Growth

Safety

Source material found negative for infectious
agents

 

 

Instructions for Use

Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

  1. Thaw Vitronectin and dilute to desired concentration using serum-free medium or PBS 。 The final solution should be sufficiently dilute so that the volume added covers the surface evenly。

  2. Add appropriate amount of diluted material to culture surface.

  3. Incubate at room temperature for approximately 1 – 2 hours.

  4. Aspirate remaining material。

  5. Rinse plates carefully with dH2O– avoid scratching bottom surface of plates.

  6. Plates are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained. 



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